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目的:通过康莱特联合顺铂对宫颈癌SiHa细胞增殖和凋亡的影响,探讨其作用机制。方法:体外培养宫颈癌Siha细胞,分别将康莱特(浓度为1,2,4,6,8 mg/mL),顺铂(浓度梯度为1.5,3,6,9,12μg/mL),单独作用于宫颈癌SiHa细胞,加药24h、48h用噻唑蓝(MTT法)检测细胞增殖情况。用流式细胞术检测康莱特组和顺铂组细胞24h凋亡率,选取合适的药物浓度(康莱特6 mg/mL,顺铂3μg/mL),进行联合用药,加药24h、48h用MTT法检测细胞增殖情况,用流式细胞术检测24h细胞凋亡率。结果:①MTT法显示加药后两组的24h、48h,宫颈癌SiHa细胞的抑制率均高于对照组(P<0.05),并且在一定程度上呈浓度和时间依赖性。②联合用药时,细胞的抑制率和凋亡率要显著高于单独用药(P<0.01)。结论:康莱特、顺铂单独或联合作用均能抑制SiHa细胞的增殖,促进其凋亡,且康莱特联合顺铂的作用要显著高于单独用药,康莱特与化疗药物联合使用可提高肿瘤细胞对化疗药物的敏感性。
Objective: To investigate the effect of Kanglaite combined with cisplatin on proliferation and apoptosis of cervical cancer SiHa cells. Methods: Siha cells were cultured in vitro. Kanglaite (1,2,4,6,8 mg / mL), cisplatin (1.5,3,6,9,12 μg / mL) Effect on cervical cancer SiHa cells, dosing 24h, 48h with thiazolyl blue (MTT method) to detect cell proliferation. Flow cytometry was used to detect the apoptotic rate of cells in KLT group and cisplatin group for 24 h. The appropriate drug concentration (KLT 6 mg / mL, cisplatin 3 μg / mL) France detected cell proliferation, flow cytometry 24h apoptosis rate. Results: (1) MTT assay showed that the inhibitory rates of SiHa cells in cervical cancer group were significantly higher than those in control group (P <0.05) at 24h and 48h after treatment, and they were both in a concentration- and time-dependent manner. ② The combination of drugs, the cell inhibition rate and apoptosis rate was significantly higher than the single drug (P <0.01). CONCLUSION: Both KLT and cisplatin can inhibit the proliferation and promote the apoptosis of SiHa cells either alone or in combination, and the effect of KLT combined with cisplatin is significantly higher than that of KLT alone. The combination of KLT and chemotherapeutic agents can increase the number of tumor cells Sensitivity to chemotherapy drugs.