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目的:建立猪肉优势腐败菌原位荧光染色检测方法。方法:将优势腐败菌韩国假单孢菌PS1接种至冷却新鲜猪肉,采用荧光染色结合激光共聚焦扫描显微镜(LCSM)技术,对肉样中的优势腐败菌实现原位荧光染色检测;优化染色条件;分析优势腐败菌在猪肉组织中的侵染方式。结果:猪肉优势腐败菌原位荧光染色双乙酸荧光素(FDA)染色最佳质量浓度为0.5 mg/mL,时间15～60 min,猪肉切片厚度可达60μm,优势腐败菌侵入猪肉肌纤维细胞的速度快于脂肪细胞,且更易从猪肉组织细胞间隙侵入。结论:利用FDA荧光染色结合LCSM技术,对猪肉中优势腐败菌原位荧光染色检测是可行的。
Objective: To establish a method for detecting in situ fluorosis of predominant spoilage pork. Methods: Pseudomonas aeruginosa PS1, a predominant spoilage bacterium, was inoculated to chilled fresh pork, and fluorescence staining and confocal laser scanning microscopy (LCSM) were used to detect the predominant spoilage bacteria in meat samples. The optimal dyeing conditions ; Analysis of dominant spoilage bacteria in pork tissue in the way of infection. Results: The optimum concentration of fluorescein diacetate (FDA) staining of predominant spoilage bacterium in pork was 0.5 mg / mL, the time was 15-60 min, the thickness of pork slice was up to 60 μm, and the rate of dominant spoilage bacteria invaded into pork myofibers Faster than fat cells, and more easily invaded from the intercellular space of pork tissue. Conclusion: It is feasible to detect the predominant spoilage bacteria in pork by fluorescence staining combined with FDA and LCSM technology.