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通过水培方法,研究了铅(Pn)和锌(Zn)及其复合胁迫对花菖蒲(Iris ensata)幼苗生长与生理指标的影响。将花菖蒲栽植于300mL培养瓶中,以1/2Hoagland营养液培养7d,在0.5mmol/L Pb+1mmol/L Pb处理下,花菖蒲幼苗地上部干重与对照组相比差异显著;其他处理与对照处理相比差异不显著;在所有Pb和Zn及其复合胁迫处理下,花菖蒲地下部干重与对照处理相比没有显著差异。除了在1mmol/L Zn处理下,花菖蒲光合色素含量与对照处理相比没有显著差异之外,其他处理的光合色素含量都比对照处理有所减少。高浓度Pb和Pb+Zn的复合胁迫对花菖蒲幼苗地上部的过氧化物酶活性诱导能力很强,在0.5mmol/LPb+1mmol/LZn复合胁迫下,花菖蒲幼苗地上部的过氧化物酶活性最高,比对照处理高81.8%;而其超氧物歧化酶活性在高浓度Pb和Zn处理下受到一定抑制;在0.5mmol/LZn胁迫下,花菖蒲地上部超氧物歧化酶活性最高,是对照处理的1.1倍。除单因子Zn处理外,花菖蒲植株地上部丙二醛的含量与对照处理相比都显著上升。研究结果说明,过氧化物酶在花菖蒲抗单因子Pb和Zn及其复合胁迫中起到了非常重要的作用,花菖蒲对Pb和Zn具有较强的耐受性,可以用于修复Pb和Zn及其复合污染的环境。
The effects of lead (Pn), zinc (Zn) and their combined stresses on the growth and physiological indices of Iris ensata seedlings were studied by hydroponics. The A. ciliata planted in 300mL flasks with 1 / 2Hoagland nutrient solution culture 7d, 0.5mmol / L Pb + 1mmol / L Pb treatment, the iris dry shoot weight above the control group, the difference was significant; other treatments and controls Compared with the control, the dry weight of understory roots did not change significantly under all treatments of Pb, Zn and their combined stress. Except 1mmol / L Zn treatment, photosynthetic pigment content of iris no significant difference compared with the control treatment, the other treatments photosynthetic pigment content than the control treatment decreased. Under the combined stress of 0.5mmol / L LPb and 1mmol / L Zn, the peroxidase activity of shoots was the highest in the shoots of A. calamus under the combined stress of high concentration of Pb and Pb + Zn. , Which was 81.8% higher than that of the control. The activities of superoxide dismutase (SOD) were inhibited under high concentrations of Pb and Zn. Under the stress of 0.5 mmol / L Zn, the activity of superoxide dismutase 1.1 times. Except for the single factor Zn treatment, the contents of malondialdehyde in shoots of A. calamus increased significantly compared with the control. The results showed that peroxidase played a very important role in the resistance of single iris to Pb and Zn and their combined stress. U. pertusa had strong tolerance to Pb and Zn and could be used to repair Pb and Zn and their complex stresses Compound pollution of the environment.