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目的：探讨ｐ５３基因突变与人食管癌淋巴结转移的关系。方法：采用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）结合银染技术，对４７例食管瘤组织中ｐ５３ 基因第５～８外显子进行了突变分析。结果：４７例食管癌标本中，ｐ５３基因第５～８外显子出现突变者２０例，其突变率为４２．６％；其中２２例伴有淋巴结转移的原发癌中１３例出现了突变（５９．１％），其与无淋巴结转移组（２８．０％，７／２５）相比有显著性差异（Ｐ＜０．０５）。结论：ｐ５３基因突变与人食管癌的转移可能关系密切。
Objective: To investigate the relationship between p53 gene mutation and lymph node metastasis in human esophageal cancer. Methods: The mutation of exons 5-8 of p53 gene in 47 cases of esophageal neoplasm was analyzed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) combined with silver staining. Results: Among 47 esophageal cancer samples, 20 cases were found mutation in exon 5 ~ 8 of p53 gene and the mutation rate was 42.6%. Of the 22 cases with primary lymph node metastasis, 13 cases showed mutation (59.1%), which was significantly different from that in the group without lymph node metastasis (28.0%, 7/25) (P <0.05). Conclusion: The mutation of p53 gene may be closely related to the metastasis of human esophageal cancer.