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目的:观察安博立酸(Ambrolic acid,AmbA)对人胃癌细胞SGC-7901增殖和凋亡的影响,初步探讨AmbA对胃癌的体外抗肿瘤活性。方法:将AmbA作用于SGC-7901细胞株,MTT法测定AmbA对SGC-7901细胞增殖的抑制率;光学显微镜观察AmbA作用引起的细胞形态变化;流式细胞仪检测AmbA作用后细胞凋亡情况及进行细胞周期分析。结果:MTT实验结果表明,AmbA对SGC-7901细胞增殖有抑制作用,其抑制效应具有时间及浓度依赖性;流式细胞仪检测分析表明,AmbA可使细胞阻滞于S期,其作用呈剂量依赖性。结论:AmbA对SGC-7901人胃癌细胞的生长具有明显的抑制作用,其作用的主要途径可能是使细胞阻滞于S期并诱导细胞凋亡。
Objective: To observe the effect of Ambrolic acid (AmbA) on the proliferation and apoptosis of human gastric cancer cell line SGC-7901 and to investigate the antitumor activity of AmbA against gastric cancer in vitro. Methods: The effect of AmbA on SGC-7901 cell line was determined by MTT assay. The cell proliferation was determined by MTT assay. The morphological changes of cells were observed by light microscopy. The apoptosis of cells was detected by flow cytometry. Perform cell cycle analysis. Results: The results of MTT assay showed that AmbA could inhibit the proliferation of SGC-7901 cells in a time and concentration-dependent manner. The results of flow cytometry showed that AmbA blocked the cells in S phase and the effect was dose-dependent Dependency. CONCLUSION: AmbA can significantly inhibit the growth of SGC-7901 human gastric cancer cells. The main effect of AmbA may be to arrest cells in S phase and induce apoptosis.