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目的：建立超临界流体萃取（ Ｓ Ｆ Ｅ） 中药何首乌中的磷脂类成分的方法。方法：采用系统观察法考察了 Ｓ Ｆ Ｅ 的提取工艺，并用反相高效液相色谱法进行分离测定。结果：在 Ｓ Ｆ Ｅ 中，通过对萃取条件的考察，确定萃取压力为３１５ Ｍ Ｐａ ，温度５０ ℃，改性剂加入量０６ ｍ Ｌ·ｇ － １ ，静态萃取时间５ ｍｉｎ 及动态萃取体积７ ｍ Ｌ。在 Ｈ Ｐ Ｌ Ｃ 中，以 Ｗａｔｅｒｓ Ｓｙｍ ｍｅｔｒｙ Ｃ１ ８ 为固定相，甲醇—１０ ％ 磷酸溶液（９０∶１０） 为流动相，检测波长２０６ ｎ ｍ 。结论： Ｓ Ｆ Ｅ Ｈ Ｐ Ｌ Ｃ 法测定何首乌中的磷脂类成分，为何首乌及其制剂的质量控制提供依据。
Objective: To establish a method for supercritical fluid extraction (SFE) of phospholipids in Radix Polygoni multiflori. Methods: The extraction process of S F E was investigated systematically and separated by reversed phase high performance liquid chromatography. RESULTS: In the S F E , through the examination of the extraction conditions, the extraction pressure was determined to be 315 M Pa, the temperature was 50 °C, the modifier dosage was 06 m L·g -1, and the static extraction time was 5 min. Dynamic extraction volume 7 m L. In the HPC, the stationary phase was Waters Symmetry C18, and the methanol-1.0% phosphoric acid solution (90:10) was used as the mobile phase. The detection wavelength was 206 nm. Conclusion: The determination of phospholipids in Radix Polygoni multiflori by S F E H P L C method provides the basis for the quality control of Radix Polygoni multiflori and its preparations.