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目的:探讨慢性乙型肝炎患者血清乙型肝炎病毒(HBV)DNA载量与肝纤维化标志物透明质酸、Ⅲ型前胶原肽、Ⅳ型胶原和层黏连蛋白水平的相关关系。方法:采用实时荧光定量PCR检测血清HBV DNA载量,采取酶促速率法检测血清ALT水平,采用化学发光检测透明质酸、Ⅲ型前胶原肽、Ⅳ型胶原和层黏连蛋白的水平。结果:1 358例慢性乙型肝炎患者血清HBV DNA载量为(4.51±1.28)copies/ml,而血清ALT、透明质酸、Ⅲ型前胶原肽、Ⅳ型胶原和层黏连蛋白的水平分别为32U/L,136ng/ml,11.27ng/ml,61ng/ml,64ng/ml,统计学分析显示血清HBV DNA载量与透明质酸水平正相关(r=0.915),但与Ⅲ型前胶原肽、Ⅳ型胶原和层黏连蛋白的水平以及ALT水平没有相关性。结论:慢性乙型肝炎患者病毒复制水平与肝细胞损伤程度程度无相关性,但与透明质酸呈正相关,因此慢性乙肝患者即使肝功能正常,也要定期检查血清HBV DNA载量和透明质酸水平。
Objective: To investigate the relationship between serum hepatitis B virus (HBV) DNA load and the levels of hyaluronic acid, type Ⅲ procollagen peptide, type Ⅳ collagen and laminin in chronic hepatitis B patients. Methods: Serum HBV DNA levels were measured by real-time fluorescence quantitative PCR. Serum ALT levels were measured by enzyme-linked immunosorbent assay. Hyaluronic acid, type Ⅲ procollagen peptide, type Ⅳ collagen and laminin were detected by chemiluminescence. Results: The serum HBV DNA load in 1 358 patients with chronic hepatitis B was (4.51 ± 1.28) copies / ml, while the levels of serum ALT, hyaluronic acid, procollagen Ⅲ, collagen Ⅳ and laminin were Statistical analysis showed that serum HBV DNA load was positively correlated with hyaluronic acid level (r = 0.915), but not with type III procollagen Peptide, type IV collagen and laminin levels and ALT levels. Conclusion: The level of virus replication in patients with chronic hepatitis B has no correlation with the degree of hepatocyte injury, but it is positively correlated with hyaluronic acid. Therefore, even if the liver function is normal in patients with chronic hepatitis B, serum HBV DNA load and hyaluronic acid Level.