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Bt蛋白能通过转Bt基因作物的秸秆还田进入土壤,进而可能会对土壤动物如蚯蚓的生长发育和生殖造成影响.为评估Bt水稻对赤子爱胜蚓的影响,本文模拟秸秆还田,在土壤中添加2.5%、5%、7.5%和10%Bt水稻(b2B138)及其同源水稻(安丰A)秸秆,分别在饲养赤子爱胜蚓7、15、30、45、60、75和90 d后观测蚯蚓的存活率、相对生长率和生殖情况,以及秸秆土壤混合物和蚯蚓体内的Cry1Ab蛋白含量.结果表明:较高还田量(7.5%和10%)Bt水稻秸秆处理对赤子爱胜蚓存活率有抑制作用;Bt水稻秸秆还田对赤子爱胜蚓的相对生长率没有不利影响;还田量为5%、7.5%和10%时,Bt水稻秸秆还田能促进蚯蚓的生殖.酶联免疫吸附测定法(ELISA)结果表明:在Bt水稻土壤混合物中,蚯蚓体内均能检测到Cry1Ab蛋白,且前者随着时间延长而显著减少.因此,还田量为2.5%和5%时,Bt水稻秸秆还田释放的Cry1Ab蛋白对赤子爱胜蚓的生长发育和生殖没有不利影响.
Bt protein can enter the soil through straw returning to the Bt gene crops, which may affect the growth and reproduction of soil animals such as earthworms.In order to evaluate the effect of Bt rice on Eisenia fetida, The soil was supplemented with 2.5%, 5%, 7.5% and 10% Bt rice (b2B138) and its homologous rice (Anfeng A) straw, After 90 days, the survival rate, relative growth rate and reproductive status of earthworm were observed, and the contents of Cry1Ab protein in the soil mixture of straw and earthworm were measured.The results showed that the treatment of Bt rice straw with higher yield (7.5% and 10% Bt rice straw returning had no adverse effect on the relative growth rate of Eisenia fetius at 5%, 7.5% and 10% of field, Bt rice straw returning could promote the reproduction of earthworms The results of enzyme-linked immunosorbent assay (ELISA) showed that the Cry1Ab protein was detectable in earthworm Bt and the former decreased significantly with time, , The Cry1Ab protein released by Bt rice straw returning to Eisenia fetida No adverse effects on development and reproduction.