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甲状腺过氧化物酶(TPO)活性的测定,概括起来主要有三种:即三碘化物法(Triiodide assay),酪氨酸碘化酶法(Tyrosine iodinase assay)及愈创木酚法(Guaiacol assay)。三碘化物法的原理是 TPO 在过量碘化物的存在下,催化Ⅰ~-→Ⅰ_2~0,Ⅰ_2和Ⅰ~-结合形成Ⅰ_3~-(三碘化物)在353nm 条件下观察其吸光度。本法的不足之处,就是过氧化氢的产生系统(葡萄糖和葡萄糖氧化酶的混合物)随着过氧化氢的消耗而使过氧化氢产生
The determination of thyroid peroxidase (TPO) activity can be summed up in three main ways: Triiodide assay, Tyrosine iodinase assay, and Guaiacol assay. . The principle of the triiodide method is that TPO catalyzes the absorption of I~-→I_2~0, I_2, and I~- to form I_3~-(triiodide) in the presence of excess iodide at 353 nm. The deficiency of this method is that the hydrogen peroxide production system (a mixture of glucose and glucose oxidase) produces hydrogen peroxide with the depletion of hydrogen peroxide.