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目的:研究复方益母黄芪汤对小鼠肿瘤生长及免疫调节作用。方法:制备移植性肝癌细胞与S180肿瘤组织生理盐水(1∶1)混悬液,每只小鼠腋窝皮下接种肝癌、S180肿瘤细胞悬液。24 h后灌胃给予复方益母黄芪汤,1次/天,连续10 d。检测其对肝癌的抑瘤作用及对S180荷瘤小鼠免疫功能的影响。结果:复方益母黄芪汤高(8.92g/kg)、中(4.46 g/kg)、低(2.23 g/kg)剂量能显著抑制肝癌及升高S180荷瘤小鼠血清IgG抗体水平,对2,4-二硝基氟苯诱导的迟发性超敏反应有增强作用,能诱导淋巴细胞转化为淋巴母细胞,提高小鼠网状内皮系统的碳粒廓清作用,促进PHA(植物血球凝集素),并能增强小鼠红细胞免疫功能。结论:复方益母黄芪汤对小鼠肝癌和S180荷瘤生长具有抑制作用,并能显著增强S180,荷瘤小鼠的细胞免疫功能。
Objective: To study the effect of Fufang Yimu Huangqi Decoction on tumor growth and immune regulation in mice. Methods: The transplanted hepatoma cells and S180 tumor tissue normal saline (1: 1) suspension were prepared. Each mouse was subcutaneously inoculated with axillary carcinoma and S180 tumor cell suspension. 24 h after gavage given Astragalus soup, once a day for 10 days. To detect its inhibitory effect on hepatocellular carcinoma and immune function of S180 tumor-bearing mice. Results: Astragalus Tang at high dosage (8.92g / kg), medium (4.46g / kg) and low dosage (2.23g / kg) could significantly inhibit the serum IgG antibody level of hepatocellular carcinoma and S180 tumor- , 4-dinitrofluorobenzene-induced delayed hypersensitivity can enhance the role of lymphocytes can be transformed into lymphoblasts, enhance the reticuloendothelial system of mice role of carbon clearance and promote PHA (Phytohemagglutinin ), And can enhance mouse erythrocyte immune function. CONCLUSION: Fufang Yimu Huangqi Decoction can inhibit the growth of mouse hepatocarcinoma and S180 tumor and enhance the cellular immune function of S180 and tumor-bearing mice.