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目的:观察帕宁方对帕金森病(PD)大鼠行为和氧化应激反应的影响。方法:运用6-羟基多巴胺(6-OHDA)立体定向脑内注射复制PD大鼠模型。将PD模型大鼠随机分为模型组、阳性对照组(美多巴0.125 g·kg-1)与帕宁方高、中、低剂量组(按生药量计为84,42,21 g·kg-1),另设同期正常组、假手术组。观察各组治疗前后大鼠旋转圈数,测定大鼠中脑黑质纹状体活性氧(ROS)、丙二醛(MDA)、谷胱甘肽(GSH)含量,谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性。结果:与模型组比较,帕宁方高、中、低剂量组大鼠旋转圈数为(237.26±19.23),(246.71±37.06),(278.81±20.09)圈,活性氧含量为(231.02±10.11),(235.87±12.76),(247.93±11.30)U·mg-1,MDA含量为(4.56±1.25),(4.70±1.17),(4.63±1.06)nmol·mg-1,均明显减少;GSH含量明显增多,为(6.20±0.81),(6.24±0.76),(6.18±0.90)mg·g-1;GSH-Px活性为(2.68±0.27),(2.59±0.34),(2.31±0.22)nU·g-1,SOD活性为(188.40±5.86),(170.87±9.80),(165.62±6.54)nU·mg-1,均明显增高,差异均有统计学意义(P<0.05)。结论:帕宁方能明显改善PD大鼠旋转行为,减轻其黑质纹状体氧化应激损伤。
Objective: To observe the effects of Panning recipe on behavior and oxidative stress in Parkinson disease (PD) rats. Methods: PD rat model was induced by stereotactic injection of 6-hydroxydopamine (6-OHDA). The PD model rats were randomly divided into model group, the positive control group (0.125 g · kg-1) and Parinitir high, medium and low dose groups (84,42,21 g · kg -1), another set the same period normal group, sham group. The number of rotations of the rats in each group was observed before and after treatment. The levels of reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase Enzyme (GSH-Px), superoxide dismutase (SOD) activity. RESULTS: Compared with the model group, the number of rotations of rats in high, medium and low doses of Panning Fang was (237.26 ± 19.23), (246.71 ± 37.06) and (278.81 ± 20.09), and the reactive oxygen species was (231.02 ± 10.11) ), (235.87 ± 12.76) and (247.93 ± 11.30) U · mg-1, and the content of MDA was (4.56 ± 1.25), (4.70 ± 1.17) and (4.63 ± 1.06) nmol · mg- (6.20 ± 0.81), (6.24 ± 0.76) and (6.18 ± 0.90) mg · g-1, respectively. The activity of GSH-Px was (2.68 ± 0.27), (2.59 ± 0.34) and (2.31 ± 0.22) nU · g-1, SOD activity was (188.40 ± 5.86), (170.87 ± 9.80), (165.62 ± 6.54) nU · mg-1, all significantly increased. Conclusion: Pavnafang can significantly improve the rotational behavior of PD rats and alleviate the oxidative stress injury of nigrostriatal system.