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目的 研究１αＯＨＤ３ 对骨质疏松的预防治疗效果和作用机理。 方法 将４０ 只雌性Ｗｉｓｔａｒ大鼠分为４组，以切除卵巢大鼠为模型，分别用０．０４ μｇ／ｋｇ·ｄ 和０．５ μｇ／ｋｇ·ｄ １αＯＨＤ３ 灌胃给药１２周。测定各组大鼠的血、尿生化指标，骨密度，生物力学和形态计量学等参数，以及骨粘连蛋白基因的表达水平。 结果 切除卵巢大鼠骨吸收和骨形成显著加强，骨密度明显降低，骨小梁结构恶化，骨生物力学性质显著降低。１αＯＨＤ３ 使大鼠骨量恢复，骨密度提高，骨小梁相对体积（ＴＢＶ）增大，并使骨生物力学性质明显提高。０．５ μｇ／ｋｇ·ｄ １αＯＨＤ３ 对骨生物力学的效果优于０．０４ μｇ／ｋｇ·ｄ １αＯＨＤ３ 的给药量。不同浓度１αＯＨＤ３ 对骨形成与骨吸收的作用不同：０．５ μｇ／ｋｇ·ｄ１αＯＨＤ３ 明显抑制骨转换；０．０４ μｇ／ｋｇ·ｄ １αＯＨＤ３ 则促进骨转换。１αＯＨＤ３ 的浓度对骨的矿化速率无显著影响。大鼠去卵巢后，骨粘连蛋白（ｏｓｔｅｏｎｅｃｔｉｎ， ＯＮ）ｍ ＲＮＡ表达水平降低，０．５ μｇ／ｋｇ·ｄ １αＯＨＤ３ 处理可恢复并促进ＯＮｍ ＲＮＡ 表达。 结论 １αＯＨＤ３ 有明显增加骨密度和骨强度的作用。高浓度１αＯＨ
Objective To study the preventive and therapeutic effects and mechanism of 1αOHD3 on osteoporosis. Methods Forty female Wistar rats were divided into four groups. The ovariectomized rats were used as the model and orally gavaged with 0.04 μg / kg · d and 0.5 μg / kg · d 1αOHD3 for 12 weeks respectively. The blood and urine biochemical parameters, bone mineral density, biomechanical and morphometric parameters and the expression of osteonectin gene were determined. Results The bone resorption and bone formation in ovariectomized rats were significantly enhanced, the bone mineral density was significantly decreased, the trabecular structure was deteriorated, and the biomechanical properties of the bone were significantly reduced. 1αOHD3 restored the recovery of bone mass, increased the BMD, and increased the relative trabecular volume (TBV). The biomechanical properties of the osteoblasts were significantly improved. The effect of 0.5 μg / kg · d 1αOHD3 on bone biomechanics was better than 0.04 μg / kg · d 1αOHD3. The effects of different concentrations of 1αOHD3 on bone formation and bone resorption were different: 0.5 μg / kg · d1αOHD3 significantly inhibited bone turnover; and 0.04 μg / kg · d 1αOHD3 promoted bone turnover. The concentration of 1αOHD3 had no significant effect on the mineralization rate of bone. After ovariectomy, the mRNA expression of osteonectin (ON) decreased, and 0.5 μg / kg · d 1αOHD3 treatment restored and promoted ONmRNA expression. Conclusion 1αOHD3 significantly increased bone mineral density and bone strength. High concentration of 1αOH