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目的探讨溶血磷脂酸(LPA)诱导HeLa-S3细胞迁移能力的机制。方法采用细胞迁移实验测定HeLa-S3细胞的迁移;Western blot检测Akt、P-Akt(Ser473)、PAK1、P-PAK1(Thr423)的表达;采用CM-H2DCFDA检测细胞ROS的生成情况。结果①20μmol·L-1LPA可使细胞内ROS的产生明显增加(P<0.05),采用NAC预处理后能明显的抑制ROS的生成;②LPA可升高P-Akt(Ser473),P-PAK1(Thr423)/PAK1水平,但采用LY294002可以抑制PAK1(Thr423)磷酸化水平的升高和细胞的迁移(P<0.01)。结论溶血磷脂酸通过上调ROS水平来诱导HeLa-S3细胞的迁移,其可能的机制是通过PI3K信号通路活化Akt/PAK1来实现的。
Objective To investigate the mechanism of lysophosphatidic acid (LPA) -induced migration of HeLa-S3 cells. Methods The migration of HeLa-S3 cells was detected by cell migration assay. The expressions of Akt, P-Akt (Ser473), PAK1 and P-PAK1 (Thr423) were detected by Western blot. The production of ROS was detected by CM-H2DCFDA. Results ①20μmol·L -1 LPA could significantly increase the intracellular ROS production (P <0.05). Pretreatment with NAC significantly inhibited the production of ROS; ② LPA increased the expression of P-Akt (Ser473), P-PAK1 (Thr423 ) / PAK1. However, LY294002 inhibited the phosphorylation of PAK1 (Thr423) and the cell migration (P <0.01). Conclusion Lysophosphatidic acid induces the migration of HeLa-S3 cells by up-regulating the level of ROS. The possible mechanism is that Akt / PAK1 is activated through the PI3K signaling pathway.