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目的:应用现代分析检测技术,定量分析附子与半夏、瓜蒌、贝母、白蔹、白及配伍前后水煎液中3种双酯型生物碱(乌头碱、中乌头碱、次乌头碱),考察其含量变化的影响因素。方法:采用高效液相色谱法,Agilent Ex-tend C18柱,乙腈-甲醇(V∶V=1∶1)-35 mmol/L乙酸铵梯度洗脱,流速0.6 mL/min,检测波长235 nm。结果:除川贝母、白蔹、法半夏外,其他各药与附子配伍后煎煮液中3种双酯型生物碱均高于附子单煎液,而其变化趋势与煎煮液的pH值有联系。结论:共煎液的pH值是影响药液中3种双酯型生物碱含量的直接因素。
OBJECTIVE: To quantitatively analyze the contents of three diester alkaloids (aconitine, mesaconitine, subacute) in the decoction of Radix Aconiti Preparata and Pinellia tuber, Fructus Fritillaria, Fritillaria, Bletilla striata, Aconitine), investigate the impact of changes in its content. Methods: High performance liquid chromatography (HPLC) with an Agilent Ex-tend C18 column with acetonitrile-methanol (V:V = 1: 1) -35 mmol / L ammonium acetate gradient at a flow rate of 0.6 mL / min and a detection wavelength of 235 nm was used. Results: Except for Fritillaria cirrhosa, Bletilla striata, Rhizoma Pinelliae, all the three diester alkaloids in the decoction of other drugs and aconite were higher than that of Fuzi decoction, and the trend was in line with the pH of decoction Value is linked. Conclusion: The pH value of decoction is the direct factor that affects the content of three diester alkaloids in liquid medicine.