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目的 观察乙型肝炎病毒 (HBV)ayw亚型转基因小鼠的肝、肾等组织的病理改变。方法 选取 15只5～ 48周龄的清洁级C57BL 6J HBV转基因小鼠及 15只C57BL 6J小鼠为对照进行病理大体观察 ,并取心、肝、脾、肺、肾、肠等组织 ,切片HE染色和免疫组化特殊染色后进行光镜观察。结果 C57BL 6J HBV转基因小鼠肝脏产生局部明显的炎症反应 :肝细胞肿胀、毛玻璃样变性、灶性坏死伴淋巴样细胞浸润 ,可见肝细胞质内嗜酸性小体 ,局部可见巨核肝细胞 ,偶见多核肝细胞 ,心、脾、肺、肾、肠等脏器未见明显的病理改变 ,免疫组化分析说明 ,血清HBsAg(乙型肝炎病毒表面抗原 )为阳性的 15只小鼠肝组织有HBsAg的存在 ,且HBsAg的分布呈胞质型 ,但不存在于脾、肺、心等组织中 ,血清HBsAg为阴性的 15只小鼠其心、肺、肝、脾、肾、肠等组织中均未见HBsAg的存在 ;发现两只 48周龄C57BL 6J HBV转基因小鼠患肝细胞癌
Objective To observe the pathological changes of liver, kidney and other tissues in hepatitis A virus (HBV) ayw transgenic mice. Methods Fifteen clean-grade C57BL 6J HBV transgenic mice aged 5 to 48 weeks and 15 C57BL 6J mice were selected for the pathological examination. The heart, liver, spleen, lung, kidney, intestine and other tissues were taken for HE staining. HE Staining and immunohistochemistry staining after special light microscopy. Results The liver of C57BL 6J HBV transgenic mice had a local inflammatory response: hepatocellular swelling, frosteoglass degeneration, focal necrosis with lymphoid infiltration, eosinophilic bodies in the cytoplasm of the liver, and local appearance of megakaryocytic hepatocytes. Liver, heart, spleen, lung, kidney, intestine and other organs showed no obvious pathological changes. Immunohistochemical analysis showed that HBsAg was positive in 15 mice with positive serum HBsAg (Hepatitis B virus surface antigen) , And the distribution of HBsAg was cytoplasmic, but not in the spleen, lung, heart and other tissues. None of the 15 mice whose serum HBsAg was negative had no changes in the heart, lung, liver, spleen, kidney, intestine and other tissues See the presence of HBsAg; two 48-week-old C57BL 6J HBV transgenic mice were found to have hepatocellular carcinoma