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众所周知,在黄酮类药物的定性分析中,把天然产物/聚乙二醇4000试剂喷射于薄层板上,使黄酮转变为荧光加成物,可以增加这类化合物检测的灵敏度和选择性,但由于TLC板分离空间有限,此法很少用于植物提取物中黄酮的定量测定。为了定量测定欧锦葵叶中的两种主要黄酮(1),(2)(参见本期文摘288-编者),作者采用的衍化反应是把展开的高效薄板浸入到一种以乙酸乙酯为溶剂的0.5％的天然产物
As we all know, in the qualitative analysis of flavonoids, the natural product/polyethylene glycol 4000 reagent is sprayed on a thin plate to convert the flavonoids into fluorescent adducts, which can increase the sensitivity and selectivity of detection of such compounds, but Due to the limited separation space of TLC plates, this method is rarely used for quantitative determination of flavonoids in plant extracts. In order to quantitatively determine the two main flavonoids in the leaves of Mallow (1), (2) (see the Abstract Editor’s Digest, 288), the derivatization reaction used by the authors was to immerse the developed high-performance sheet in an ethyl acetate solvent. 0.5% natural product