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本文用荧光光谱方法检测了HPD对妊娠中期家兔胎盘和胎儿的亲和力。实验用健康母兔4—5公斤/只,用氯化血红素经溴化加成水解提取血卟啉,然后按Liposin方法制备HPD,参照Henderson方法配制注射液。母兔按交配当天算起的第15天(妊娠中期)以每公斤体重从耳静脉注入20mgHPD,经24小时供实验用。首先测定HPD在血清中的荧光激发谱和发射谱;从未经注入HPD孕兔耳静脉采血2.5ml,同时加入0.5ml3.2％柠檬酸三钠溶液抗凝,离心取其上清液。在每ml清血中加入5微克HPD,置PMQ_3型荧光分光光度计测得其激发峰
In this paper, fluorescence spectrometry was used to detect the affinity of HPD to the placenta and fetus in the second trimester of pregnancy. Experimental use of healthy female rabbits 4-5 kg / hematin hemin by bromine hydrolysis hydrolyzate Hematoporphyrin, and then prepared according to Liposin HPD, formulated according to Henderson injection. The females were injected with 20 mg HPD per ear body weight from the ear vein on the 15th day of gestation (the second trimester) on the day of mating, and were tested for 24 hours. Firstly, the fluorescence excitation spectrum and emission spectrum of HPD in serum were determined. 2.5 ml of 2.5 ml 3.2% trisodium citrate solution was collected from the ear vein of HPD pregnant rabbits, and the supernatant was obtained by centrifugation. Add 5 micrograms of HPD to each ml of blood and set the PMQ_3 fluorescence spectrophotometer to measure its excitation peak