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采用时间分辨荧光光谱技术研究了在双光子激发下不同尺寸的量子点与罗丹明B之间的荧光共振能量转移.研究结果表明,在800 nm的双光子激发条件下,体系间能量转移效率随着供体吸收光谱与受体荧光光谱的光谱重叠程度增加而增加;理论分析表明,供体和受体间的Frster半径增加是导致其双光子能量转移效率增大的物理原因.同时,研究了罗丹明B浓度对荧光共振能量转移效率的影响.研究结果表明,量子点的荧光寿命随着罗丹明B浓度的增加而减小;量子点与罗丹明B之间的荧光共振能量转移效率随着罗丹明B浓度的增加而增加;当罗丹明B浓度为3.0×10-5mol·L-1时,双光子荧光共振能量转移效率为40.1%.
Fluorescence resonance energy transfer between quantum dots with different sizes and rhodamine B under two-photon excitation was studied by using time-resolved fluorescence spectroscopy.The results show that the energy transfer efficiency between two quantum dots under 800 nm two-photon excitation The spectral overlap between the donor absorption spectrum and the fluorescence spectrum of the acceptor increases with the increase of the spectral overlap. The theoretical analysis shows that the increase of F rster radius between donor and acceptor is the physical reason for the increase of the energy transfer efficiency of two- The effect of rhodamine B concentration on the fluorescence resonance energy transfer efficiency was studied.The results show that the fluorescence lifetime of quantum dots decreases with the increase of rhodamine B concentration.The fluorescence resonance energy transfer efficiency between quantum dots and rhodamine B With the increase of rhodamine B concentration. When rhodamine B concentration was 3.0 × 10-5 mol·L-1, the energy transfer efficiency of two-photon fluorescence resonance was 40.1%.