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恶性转化的金仓鼠乳鼠肺成纤维细胞BHLB_4,在2 mmol/L丁酸长期处理下部分表型发生逆转,明显地趋于正常。用间接免疫荧光标记法发现BHLB_4细胞表面纤维粘连蛋白丧失,而丁酸处理可使其在细胞膜表面重新附着,成为纤维索状分布。进一步分离测定金仓鼠细胞表面Fn分子量为250 kDa,在正常对照和丁酸逆转的细胞表面含量相对较高。实验揭示了细胞表面Fn在分布和数量上的变化,同金仓鼠细胞的转化或逆转形态有较密切的关系,可以方便地作为我们初步衡量细胞是否转化的一个较为客观的指标。
The malignant hamster pups’ lung fibroblasts BHLB_4 were reversed and their partial phenotypes reversed after 2 mmol/L butyric acid treatment for a long time. The loss of fibronectin on the surface of BHLB_4 cells was detected by indirect immunofluorescence labeling, and butyrate treatment reattached to the surface of the cell membrane and became a fibroform-like distribution. The molecular weight of Fn on the surface of gold hamster cells was further determined to be 250 kDa, which was relatively high in the normal control and butyrate-reversed cell surface. The experiment revealed changes in the distribution and number of Fn on the cell surface, which is closely related to the transformation or reversion of the morphology of the gold hamster cells, and can be conveniently used as a more objective indicator of whether or not cells are transformed initially.