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用封闭群大耳白家兔雌性24只,随机分为三组。对照组动物不加任何处理,发热组和电针组动物i.v.内毒素(ET)引起发热反应,发热组动物i.v.ET后80min体温上升了1.18℃,而电针组动物体温仅上升了0.35℃,比较两组动物的体温上升高度差异非常显著(P<0.001)。给ET后80min处死动物,观察动物丘脑下部腺苷酸环化酶(AC)及磷酸二酯酶(PDE)活性的变化。发热组动物AC活性增强和对照组比较差异非常显著(P<0.001);电针组动物AC活性减弱和对照组比较差异非常显著(P<0.01)。发热组和电针组动物PDE活性均增强与对照组比较差异均非常显著(P<0.001)。实验结果表明,发热组AC和PDE的活性均增强,而电针组AC活性明显减弱,PDE的活性增强。作者推论:家兔ET性发热时丘脑下部环一磷酸腺苷(cAMP)含量增加是由于AC活性增强使cAMP生成增加,电针组动物丘脑下部cAMP含量减少是由于AC活性减弱,PDE活性增强结果cAMP生成减少而分解增加所致。
Twenty-four female white rabbits were divided into three groups randomly. Animals in the control group were treated without any treatment. The endotoxin (ET) in fever group and EA group caused fever reaction. The body temperature in fever group increased by 1.18 ℃ at 80 minutes after ivET, while the body temperature in EA group only increased by 0.35 ℃, The differences in body temperature rise between the two groups were significant (P <0.001). Animals were sacrificed at 80 min after ET to observe the change of adenosine cyclase (AC) and phosphodiesterase (PDE) activity in the hypothalamus of animals. Compared with the control group, the AC activity in fever group was significantly increased (P <0.001). The AC activity in the EA group was significantly lower than that in the control group (P <0.01). The activity of PDE in fever group and electroacupuncture group were significantly higher than those in control group (P <0.001). The experimental results showed that both AC and PDE activity in fever group were increased, while AC activity in EA group was significantly weakened and PDE activity was enhanced. The authors conclude that increased cAMP content in the hypothalamus in ET-induced fever in rabbits is due to an increase in cAMP production due to increased AC activity, a decrease in cAMP content in the hypothalamus of EA rats due to reduced AC activity and enhanced PDE activity Decreased cAMP production caused by increased decomposition.