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目的 :研究通脑灵对 N-甲基 - D-门冬氨酸 (NMDA)介导的神经细胞内游离钙离子 ([Ca2 + ]i)浓度的影响。方法 :应用标记示踪法 ,以 Fura- 2 / AM为荧光指示剂观察通脑灵对 NMDA介导的神经细胞内游离钙离子浓度的影响。结果 :5 0 0μm ol· L- 1 NMDA可使神经细胞内 [Ca2 + ]i明显增高 ,与对照组比较有显著性差异 (P<0 .0 1)。通脑灵可阻止 NMDA介导的神经细胞内 [Ca2 + ]i超载。结论 :通脑灵通过抑制 NMDA介导的钙离子超载而发挥对神经细胞的保护作用
Objective : To study the effect of Tongnaoling on N-methyl-D-aspartate (NMDA)-mediated intracellular free calcium ion ([Ca2 + ]i) in neurons. Methods : The labeling tracer method was used to observe the effect of Tongnao Ling on NMDA-mediated free calcium ion concentration in neurons with Fura-2 / AM as fluorescent indicator. RESULTS : The increase of [Ca2 +]i in nerve cells was significantly increased by 500 μmol·L·L-1 NMDA, which was significantly different from that in the control group (P<0.01). Tongnaoling can prevent NMDA-mediated [Ca2+]i overload in nerve cells. Conclusion :Tong Nao Ling exerts protective effects on nerve cells by inhibiting NMDA-mediated calcium overload