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我们采用改良Hp培养基(HBSV培养基)对Hp进行自然诱导,发现Hp球形变异体在含有适量蛋黄和血清的培养中容易返祖及传代,部分培养超过5天的菌落中有长丝体形成,或在球变体两侧形成长短不等的菌丝。长丝体变异的菌落与一般无异,但形态多较大,长丝体约为典型菌的3～10倍以上,菌体弯曲不规则或缠绕,粗细不均,胞壁缺陷,但可在普通渗透压的平板上生长。长丝体不稳定,接种于Hp或HBSV培养基返祖成典型的“S”形Hp。本研究提出这样的假说:Hp变异由典型“S”形→球变→长丝体和巨球体变异→返祖成典型Hp,Hp的这种变异可能存其传染和感染复发中起重要作用。
We used modified Hp medium (HBSV medium) to naturally induce Hp and found that Hp spheroids were easily returned to ancestors and passaged in cultures containing appropriate amount of egg yolk and serum. Some colonies cultured for more than 5 days had filament formation , Or in the ball on both sides of the formation of varying lengths of mycelium. Filament changes in the colony and the general body is no different, but more form larger filament body is about 3 to 10 times the typical bacteria, bacteria bent irregular or winding, uneven thickness, cell wall defects, but in Ordinary osmotic pressure on the growth of the plate. Filament body instability, inoculation of Hp or HBSV medium into a typical “S” shaped Hp. This study proposed the hypothesis that the Hp variation consists of a typical “S” shape → a spherochyx → a filamentous body and a giant spheroid → an ancestor into a typical Hp that may play an important role in infection and infection recurrence.