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Extraction of AchE, relationship between substrate and enzyme concentration, and rninhibition effects of the agrochemicals to AchE are discussed in this paper. Through the rernsearch, the proper AchE concentration for hydrolysis of 1 ml 1mmoL/L substrate and I50 valrnues of the agrochemicals to AchE are decided. It is proved that Asch-DTNB method is a rnrapid test tool for agrochemical residues in fruits and vegetables. A rapid test card has rnbeen developed with sensitivity of 0.05mg/L.