高迁移率族蛋白B1(High Mobility Group Box 1,HMGB1)参与炎症反应的分子基础和信号过程一直是国内外的研究热点。近年来已鉴定出HMGB1的一些细胞表面受体,但仍未能完全解释其信号过程。本研究通过构建HMGB1与增强型绿色荧光蛋白的融合蛋白并加入到体外培养哺乳动物细胞上清中, It has been a hot research topic both at home and abroad that the high mobility group box 1 (HMGB1) participates in the molecular basis and signaling process of inflammatory response. In recent years, some cell surface receptors of HMGB1 have been identified, but their signaling processes have not yet been fully elucidated. In this study, the fusion protein of HMGB1 and enhanced green fluorescent protein was constructed and added to the supernatant of mammalian cells cultured in vitro,