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目的观察补肾益髓方促进实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)小鼠髓鞘再生的作用。方法 80只雌性C57BL/6小鼠随机分为正常对照、模型、醋酸泼尼松及补肾益髓方组。以髓鞘少突胶质细胞糖蛋白(myelin oligodendroglia glycoprotein,MOG)35-55诱导小鼠EAE模型。治疗组小鼠每天给予相应药物灌胃治疗,共40天,醋酸泼尼松为阳性对照药物。每天观察动物的一般情况,记录小鼠神经功能评分,统计小鼠的发病率、潜伏期、病程和疾病负荷等。取小鼠脑和脊髓经固蓝染色观察其髓鞘变化,免疫组化法测定髓鞘再生相关蛋白硫酸软骨素蛋白多糖2胶质细胞和髓鞘碱性蛋白(myelin basic protein,MBP)的表达。结果补肾益髓方能够延长小鼠发病潜伏期、缩短EAE病程、降低小鼠疾病负荷(P<0.05),减少小鼠脑和脊髓内炎细胞浸润和髓鞘脱失;本方还可促进NG2和MBP蛋白的表达(P<0.01)。结论补肾益髓方对EAE小鼠有神经保护作用,并能减轻髓鞘损伤和促进髓鞘修复。
Objective To observe the effect of Bushen Yisui Fang on myelin regeneration in mice with experimental autoimmune encephalomyelitis (EAE). Methods Eighty female C57BL / 6 mice were randomly divided into normal control, model, prednisone acetate and Bushen Yisui Fang. Mouse EAE model was induced by myelin oligodendroglia glycoprotein (MOG) 35-55. The mice in the treatment group were treated with the corresponding drugs daily for 40 days and prednisone acetate as the positive control drug. Observed the general situation of animals daily, recorded the score of neurological function in mice, statistics of the incidence of mice, latency, duration and disease load. The myelin changes of the mouse brain and spinal cord were observed by solid blue staining, and the expression of myelin basic protein (MBP) and myelin regeneration related protein 2 . Results Bushen Yisui Fang could prolong the incubation period of mice, shorten the course of EAE, reduce the disease burden in mice (P <0.05), reduce the infiltration of inflammatory cells and demyelination in mice brain and spinal cord. MBP protein expression (P <0.01). Conclusion Bushen Yisui Fang has neuroprotective effects on EAE mice, and can reduce myelin damage and promote myelin sheath repair.