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应用改良的 Chelex- 10 0滤纸处理法对广东省中山市 40例间日疟病人滤纸血标本进行处理后经 PCR扩增 ,根据扩增结果判别 CSP基因型。结果 40例全部为 PV- 型温带族 ,未发现有 PV- 型热带族和 PV- 型病例。表明广东省中山市间日疟病人所感染的疟原虫优势虫株为 PV- 型温带族。
Filter paper blood samples from 40 malaria patients were treated with modified Chelex-10 filter paper in Zhongshan City, Guangdong Province. PCR-based amplification was used to determine CSP genotype according to the amplification results. Results All 40 cases were PV-type temperate families, and no PV-type tropical family and PV-type cases were found. It showed that the predominant parasites of Plasmodium vivax in Zhongshan City, Guangdong Province were PV-type temperate families.