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着色性干皮病是先天性DNA修复功能缺陷的常染色体隐性遗传疾病。是研究人类DNA修复系统与肿瘤发生机理的理想实验模型。本文报告的XP4SH细胞系来源于XP患者皮肤,经体外培养表现为成纤维细胞的形态和生长特性,染色体众数为2n=46,双着丝粒染色体为其畸变的特征,经紫外线诱发的SCE率显著增高,而UDS水平则明显低下,细胞融合基因互补分析证实该细胞系归属于A互补群。
Chromic xeroderma is an autosomal recessive disease that congenital DNA repair defects. Is to study human DNA repair system and tumor mechanism of the ideal experimental model. The XP4SH cell line reported in this paper is derived from the skin of XP patients. The morphological and growth characteristics of the fibroblasts are shown in vitro. The chromosome number is 2n = 46, and the dicentric chromosome is the characteristic of aberration. The UV-induced SCE Rate was significantly higher, while the UDS level was significantly lower, cell fusion gene complementation analysis confirmed that the cell line belongs to the A complementary group.