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背景:神经肽Y能神经元分布于大脑皮质、尾壳核、边缘系统、丘脑、脑干等多个部位,与运动的学习、记忆及高级整合功能有关;小脑具有通过学习而重新组合的能力,研究大鼠小脑皮质内神经肽Y阳性神经元的形态与分布,可对小脑皮质运动学习的形态学基础有所了解。目的:研究大鼠小脑皮质内神经肽Y免疫阳性神经元的形态与分布,探讨神经肽Y能神经元与小脑运动学习、记忆的关系。设计:以动物为研究对象,单一样本研究。单位:新乡医学院解剖学教研室、病理生理教研室和形态中心。材料:实验于2001-07/12在新乡医学院形态中心进行。清洁级SD大鼠10只,雌雄不限,体质量100～200g。方法:腹腔麻醉后,升主动脉灌注固定,开颅取小脑,入相同固定液中后固定48h,常规石蜡包埋;沿小脑矢状面作连续切片。神经肽Y免疫组化染色,阳性对照采用大鼠大脑切片;阴性对照用正常小牛血清替代第一抗体,0.01mol/L磷酸盐缓冲液替代第二抗体。光镜下观察,显微照相。主要观察指标:观察大鼠小脑皮质内神经肽Y能神经元的形态与分布。结果:大鼠小脑内神经肽Y免疫阳性神经元分布在皮质的Purkinje细胞层,胞体呈梨形或烧瓶状,细胞核清晰不染色,轴突伸向颗粒层,树突呈网状伸向分子层,突起未见染色。分子层和颗粒层未见阳性细胞,但可见神经纤维的存在。结论
Background: Neuropeptide Y neurons are distributed in many parts of the cerebral cortex, caudate putamen, limbic system, thalamus and brain stem, which are related to motor learning, memory and advanced integration functions. The cerebellum has the ability to regroup by learning , To study the morphology and distribution of neuropeptide Y-positive neurons in the cerebellar cortex of the rat, may understand the morphological basis of cerebellar cortical motor learning. OBJECTIVE: To study the morphology and distribution of neuropeptide Y immunoreactive neurons in the cerebellar cortex of rats and to explore the relationship between neuropeptide Y neurons and cerebellum motor learning and memory. Design: animal as a research object, a single sample study. Unit: Department of Anatomy Xinxiang Medical College, Department of Pathophysiology and Morphology Center. MATERIALS: The experiment was performed at Morphological Center of Xinxiang Medical College from July 2001 to December 12, 2001. Clean-grade SD rats 10, male or female, body weight 100 ~ 200g. Methods: After intraperitoneal anesthesia, the ascending aorta was perfused and fixed. The cranium was taken from the cerebellum and fixed in the same fixative liquid for 48 hours. The paraffin was embedded in paraffin and serially sliced along the sagittal plane of the cerebellum. Neuropeptide Y immunohistochemical staining, the positive control rat brain sections; negative control with normal fetal calf serum instead of the first antibody, 0.01mol / L phosphate buffer instead of the second antibody. Light microscopy, photomicrography. MAIN OUTCOME MEASURES: The morphology and distribution of neuropeptide Y neurons in the cerebellar cortex of rats were observed. Results: The neuropeptide Y immunoreactive neurons in the cerebellum were distributed in the layer of Purkinje cells in the cortex. The cell bodies were pear-shaped or flask-like. The nuclei were clear and not stained. The axons extended to the granular layer. The dendrites extended to the molecular layer , Protruding no staining. No positive cells were observed in the molecular and granular layers, but the presence of nerve fibers was observed. in conclusion