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目的:研究甲状腺组织半乳糖凝素3(Galectin-3)和垂体瘤转化基因(PTTG)在甲状腺良恶性疾病中的表达特点及鉴别诊断中的应用价值。方法:应用免疫组织化学Envision法检测正常甲状腺组织10例、甲状腺滤泡性腺瘤(腺瘤)13例、滤泡性癌14例、乳头状癌40例中单克隆抗体Galectin-3、PTTG的表达。结果:甲状腺病变中Galectin-3和PTTG表达均位于细胞质;Galectin-3、PTTG的表达在甲状腺腺瘤中74.62%为阴性,而滤泡性癌阳性明显增加,乳头状癌90%为中、强阳性,Galectin-3和PTTG在腺瘤阳性表达率分别为15.38%(2/13)和15.38%(2/13),在滤泡性癌分别为64.29%(9/14)和57.14%(8/14),在乳头状癌分别为95%(38/40)、90%(36/40);Galectin-3和PTTG在正常甲状腺组织、甲状腺腺瘤与恶性病变(滤泡性癌、乳头状癌)表达差异均有统计学意义(P均<0.05);在恶性肿瘤(滤泡性癌和乳头状癌)间表达差异亦有统计学意义。10例正常甲状腺组织Galectin-3和PTTG均不表达,腺瘤中仅有1例表达,腺瘤2者均阳性表达为7.69%,滤泡性癌为21.43%,乳头状癌为100%。结论:Galectin-3和PTTG免疫组化联合检测对甲状腺病变的诊断、鉴别诊断具有较高的实用价值。
Objective: To study the expression of Galectin-3 and pituitary tumor transforming gene (PTTG) in thyroid gland in malignant and benign thyroid diseases and its differential diagnostic value. Methods: Immunohistochemical Envision method was used to detect the expression of Galectin-3 and PTTG in 10 cases of normal thyroid tissue, 13 cases of thyroid follicular adenoma (adenoma), 14 cases of follicular carcinoma and 40 cases of papillary carcinoma . Results: The expression of Galectin-3 and PTTG in thyroid lesions were all located in the cytoplasm. The expression of Galectin-3 and PTTG was negative in 74.62% of thyroid adenomas, while the positive follicular carcinoma was significantly increased. 90% of papillary carcinomas were moderate and strong The positive expression rates of Galectin-3 and PTTG in adenomas were 15.38% (2/13) and 15.38% (2/13), respectively, and those in follicular carcinomas were 64.29% (9/14) and 57.14% / 14) and 95% (38/40) and 90% (36/40) respectively in papillary carcinoma. Galectin-3 and PTTG in normal thyroid tissue, thyroid adenoma and malignant lesions (follicular carcinoma, papillary (P <0.05). There was also a significant difference in the expression between malignant tumors (follicular carcinoma and papillary carcinoma). The expression of Galectin-3 and PTTG in 10 cases of normal thyroid tissue were not expressed. Only 1 of them was expressed in adenoma. The positive expression of adenoma was 7.69%, follicular carcinoma was 21.43% and papillary carcinoma was 100%. Conclusion: Galectin-3 and PTTG immunohistochemical detection of thyroid lesions in the diagnosis of differential diagnosis has a high practical value.