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目的确定已获得的四株榛子主要过敏原Cor h1单克隆抗体识别的抗原表位区域。方法构建谷胱甘肽疏基转移酶(GST)与Cor h1不同截短体的表达载体并表达GST-Cor h1融合蛋白,采用ELISA和Western blot检测单抗与不同融合蛋白的反应,并结合DNAstar表位分析软件推知不同单抗的抗原识别表位区域。结果确定了四株Cor h1单抗的抗原识别表位区域,分别是4G7和2H3抗体的抗原表位位于Cor h1第120～126位氨基酸,5F2和1G4抗体的抗原表位位于Cor h1第43～52位氨基酸。结论四株Cor h1单抗的抗原识别表位区域的确定,为Cor h1的进一步研究和食品安全检测奠定了基础。
OBJECTIVE: To determine the epitope regions recognized by Corh1 monoclonal antibody of the four hazelnut allergens that have been obtained. Methods GST-Cor h1 fusion protein was constructed by using GST-Cor h1 truncated truncated expression vector. ELISA and Western blot were used to detect the binding of mAbs to different fusion proteins. The binding of DNAstar Epitope analysis software deduced that different monoclonal antibodies antigen recognition epitope region. Results The antigenic epitope regions of the four monoclonal antibodies to Corh1 were determined. The antigenic epitopes of the 4G7 and 2H3 antibodies were located at amino acids 120 to 126 of Cor h1, and the epitopes of the 5F2 and 1G4 antibodies were located at the amino acid positions 43-45 of Cor h1 52 amino acids. Conclusion The identification of the epitopes of the four monoclonal antibodies against Cor h1 has laid the foundation for further research on Cor h1 and food safety testing.