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目的:研究口腔扁平苔藓(OLP1)患者Th1型和Th2型细胞因子的表达及临床意义。方法:选取2013年1月至2014年3月于我院就诊的21例充血糜烂型及14例光滑型OLP患者为研究对象,18例正常人为对照组,采用密度梯度离心法对各组外周血单个核细胞进行分离,酶联免疫吸附剂测定(ELISA)法对各组外周血单个核细胞中的IL-4和IFN-gamma的表达进行检测,逆转录-聚合酶链反应法对各组血清中IL-4 m RNA和IFN-gamma m RNA的表达进行检测。结果:与正常对照组相比,OLP患者IL-4m RNA及蛋白的表达均增高,而IFN-gamma m RNA及蛋白的表达则降低,差异均有显著统计学意义(均P<0.01)。充血糜烂型及光滑型OLP患者组间比较发现,IL-4 m RNA和IFN-gamma m RNA及蛋白的表达差异无统计学意义(P>0.05)。结论:OLP发病机制与Th1与Th2的表达失衡有关,为临床治疗提供参考。
Objective: To investigate the expression of Th1 and Th2 cytokines in patients with oral lichen planus (OLP1) and its clinical significance. Methods: Twenty-one patients with congestive-erosive and 14 patients with OLP who were treated in our hospital from January 2013 to March 2014 were selected as study subjects and 18 normal controls as control group. The density of peripheral blood The expression of IL-4 and IFN-gamma in peripheral blood mononuclear cells of each group were detected by enzyme linked immunosorbent assay (ELISA), and the expression of IL-4 and IFN-gamma in peripheral blood mononuclear cells of each group were detected by reverse transcriptase-polymerase chain reaction IL-4 m RNA and IFN-gamma m RNA were detected. Results: Compared with the normal control group, the expression of IL-4m RNA and protein in OLP patients was increased, while the expression of IFN-gamma m RNA and protein was decreased in OLP patients (all P <0.01). There was no significant difference in the expression of IL-4 mRNA and IFN-gamma m RNA and protein in patients with congestive and erosive OLP (P> 0.05). Conclusion: The pathogenesis of OLP is related to the unbalance of the expression of Th1 and Th2, providing a reference for clinical treatment.