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硒是人体内必须的微量元素,近年来防癌抗癌作用受到了广泛关注。本研究探索了甲基硒酸对肺癌细胞增殖凋亡的影响及其机制,为后续研究提供理论依据。1资料与方法1.1材料肺癌细胞株A549由泰安医学院提供。甲基硒酸购自Sigma公司;PBS、RPMI1640培养基购自美国Gibco公司;胎牛血清购自成都哈利生物有限公司;胰蛋白酶购自北京鼎国生物技术有限公司;CCK8
Selenium is a necessary trace element in human body. In recent years, anti-cancer and anti-cancer effects have received widespread attention. This study explored the impact of methyl selenite on proliferation and apoptosis of lung cancer cells and its mechanism for the follow-up study to provide a theoretical basis. 1 Materials and Methods 1.1 Materials Lung cancer cell line A549 provided by the Tai’an Medical College. Methylselenoacid was purchased from Sigma Company; PBS, RPMI1640 medium was purchased from Gibco, USA; fetal bovine serum was purchased from Chengdu Halle Biotech Co., Ltd.; trypsin was purchased from Beijing Dingguo Biotechnology Co., Ltd.; CCK8