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[目的]建立颠茄高频再生体系及筛选卡那霉素(Kan)抗性。[方法]以颠茄叶片及腋芽为外植体,研究了培养基中6-BA和NAA不同配比对其不定芽分化的影响以及叶片不定芽对Kan的敏感性。[结果]MS+4.5mg/L6-BA+0.2mg/LNAA为叶片不定芽分化的最佳培养基,不定芽分化率达100%,在1.0cm×1.0cm叶块上的不定芽分化数平均达5.85;MS+3.0mg/L6-BA+0.1mg/LNAA为腋芽不定芽分化的最适培养基,不定芽分化率达100%,每个腋芽不定芽分化平均数为4～8个;400.0mg/LKan为颠茄叶片遗传转化的最佳筛选浓度。[结论]为颠茄无菌苗的快速繁殖以及基于叶盘法的遗传转化奠定了基础。
[Objective] The research aimed to establish a high-frequency regeneration system of Belladonna and screen Kan resistance. [Method] With the belladonna leaf and axillary bud as explants, the effects of different ratios of 6-BA and NAA on the differentiation of adventitious buds and the sensitivity of adventitious buds to Kan were studied. [Result] MS + 4.5 mg / L 6-BA + 0.2 mg / L NAA was the best medium for adventitious bud differentiation, and the adventitious bud differentiation rate was 100%. The average number of adventitious bud differentiation on 1.0 cm × 1.0 cm leaves Up to 5.85. MS + 3.0 mg / L 6-BA + 0.1 mg / L NAA was the most suitable medium for adventitious bud differentiation of axillary buds. The rate of adventitious bud differentiation was 100%, and the average number of adventitious buds per axillary bud was 4 ~ 8. mg / LKan belladonna leaf genetic transformation of the best screening concentration. [Conclusion] The study laid the foundation for the rapid propagation of sterile belladonna plants and the genetic transformation based on the leaf disc method.