论文部分内容阅读
以鸡胚背根神经节无血清培养及大鼠子鼠脑神经细胞无血清培养作为模型 ,检测以新生小牛脑为原料制备的低分子脑提取物对神经细胞突起生长的作用。结果表明 :(1)低分子脑提取物可促进鸡胚背根神经节神经突起的生长 ,加入低分子脑提取物 48h后 ,神经节周围长出突起 ,随着时间的推移 ,神经突起生长得长而密 ,相互交织在一起。 (2 )借助图像分析仪 ,以大鼠子鼠脑神经细胞神经突起平均数及神经突起平均长度为指标 ,观察到低分子脑提取物能促进神经细胞神经突起生长。低分子脑提取物经HPLC分析 ,在 2 80nm部分可分为 5个组分 ,究竟是哪一组分或哪几组分起促神经突起生长作用 ,尚需进一步研究。
Serum-free culture of chick embryo dorsal root ganglion and serum-free culture of rat brain neurons were used as models to detect the effect of low molecular weight brain extracts prepared from newborn calf brain on neurite outgrowth. The results showed that: (1) low molecular brain extract can promote the growth of chick embryo dorsal root ganglion neurite growth, adding low molecular brain extract 48h, the ganglion grows protuberances, with the passage of time, neurite growth Long and dense, intertwined. (2) With the help of image analyzer, taking the average number of neurons in rat brain neurons and the average length of neurites as indexes, it was observed that low molecular brain extracts can promote the neurite outgrowth. Low molecular brain extracts by HPLC analysis, in the 2 80nm part can be divided into five components, which one or which components play a role in promoting neurite growth, still need further study.