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背景长期过量饮用啤酒有可能会引起体内组织或血清酶活性的变化。目的观察啤酒的饮用量与大鼠脂肪合成与分解代谢相关酶活性的关系。设计随机对照动物实验。单位泰山医学院基础医学研究所。材料实验于2004-12/2005-02在泰山医学院基础医学研究所完成。选择SD大鼠60只,分为6组,每组10只。按体质量每天灌服啤酒量分为9m L/kg,18m L/kg,27mL/kg,36m L/kg及45m L/kg组;对照组大鼠食水自由,不灌服啤酒。方法各组大鼠连续喂饲1周后,麻醉处死大鼠,采取血样,留取肝脏、皮下脂肪、肠系膜脂肪组织以及腓肠肌,分别进行生化分析和酶活性的测定。主要观察指标①喂饲1周后各组大鼠体质量、血糖、胰岛素以及血脂水平。②喂饲1周后各组大鼠肝脏和脂肪组织酶活性。③喂饲1周后各组大鼠激素敏感脂肪酶和脂蛋白脂肪酶活性。结果纳入60只大鼠全部进入结果分析,无脱失。①喂饲1周后各组大鼠体质量及生化指标水平比较啤酒36m L/kg组大鼠体质量、血清游离脂肪酸、高密度脂蛋白胆固醇、肝脏三酰甘油及肝脏胆固醇含量均显著高于对照组(χ2=19.44～20.01,P<0.01)。②喂饲1周后各组大鼠肝脏和脂肪组织酶活性比较啤酒36m L/kg组大鼠肝脏、皮下脂肪组织和肠系膜组织的肝脏微粒体三酰甘油转换蛋白、磷脂酰磷酸水解酶、苹果酸酶和葡萄糖-6-磷酸脱氢酶的活性均显著高于对照组(χ2=15.02～16.00,P<0.05)。③喂饲1周后各组大鼠激素敏感脂肪酶和脂蛋白脂肪酶活性比较啤酒36m L/kg组大鼠腓肠肌激素敏感脂肪酶活性显著低于对照组(P<0.01),但皮下脂肪组织的激素敏感脂肪酶活性显著高于对照组(P<0.01)。啤酒36m L/kg组在肠系膜脂肪、皮下脂肪及腓肠肌组织中脂蛋白脂肪酶活性均显著高于其他啤酒组和对照组(χ2=19.00～20.00,P<0.01)。结论每日灌服一定量的啤酒(36m L/kg)可促进大鼠肝脏合成和转运三酰甘油的能力,脂肪组织如肠系膜脂肪组织的脂质合成和储存增多,外周组织如肌肉组织和皮下脂肪组织的脂肪分解和动员也增加,最终导致体质量增长。
Background Long-term overdrinking beer may cause changes in tissue or serum enzyme activity in the body. Objective To observe the relationship between beer consumption and enzyme activity related to fat synthesis and catabolism in rats. Design randomized controlled animal experiments. Unit Taishan Medical College Institute of Basic Medicine. Materials experiment was performed at Institute of Basic Medical Sciences, Taishan Medical College from December 2004 to February 2005. 60 SD rats were selected and divided into 6 groups with 10 in each group. According to the body weight, the amount of beer fed daily was divided into 9m L / kg, 18m L / kg, 27mL / kg, 36m L / kg and 45m L / kg groups; Methods Rats in each group were fed continuously for 1 week. Rats were sacrificed by anesthesia. Blood samples, liver, subcutaneous fat, mesenteric adipose tissue and gastrocnemius muscle were collected for biochemical analysis and enzyme activity determination respectively. MAIN OUTCOME MEASURES ① The body weight, blood glucose, insulin and blood lipid levels of rats in each group after 1 week of feeding. ② After 1 week of feeding, the activity of liver and adipose tissue of rats in each group was increased. ③ After 1 week of feeding, the hormone-sensitive lipase and lipoprotein lipase activity of rats in each group were significantly increased. Results All 60 rats were included in the result analysis without loss of result. ①Comparison of body weight and biochemical indexes of rats in one group after feeding for one week The body weight, serum free fatty acid, high density lipoprotein cholesterol, liver triglyceride and liver cholesterol of 36m L / kg group were significantly higher than those of the control Control group (χ2 = 19.44 ~ 20.01, P <0.01). ② Enzyme activity of liver and adipose tissue of rats in each group after 1 week of feedingCompared with liver microsomes of triglyceride and phosphatidylphosphate hydrolase in liver, subcutaneous adipose tissue and mesentery in 36 m L / kg of beer, The activities of acid phosphatase and glucose-6-phosphate dehydrogenase were significantly higher than that of the control group (χ2 = 15.02-16.00, P <0.05). ③ Comparison of hormone-sensitive lipase and lipoprotein lipase activity in rats after 1 week of feeding Gastrocnemius hormone-sensitive lipase activity in 36 m L / kg beer group was significantly lower than that in control group (P <0.01), but subcutaneous adipose tissue The hormone-sensitive lipase activity was significantly higher than that of the control group (P <0.01). Beer 36m L / kg group in mesenteric fat, subcutaneous fat and gastrocnemius tissue lipoprotein lipase activity were significantly higher than other beer group and control group (χ2 = 19.00 ~ 20.00, P <0.01). CONCLUSION: Daily administration of a certain amount of beer (36 m L / kg) promoted the synthesis of hepatic triglycerides in rats and increased the lipid accumulation and accumulation in adipose tissue such as mesentery adipose tissue. Peripheral tissues such as muscle tissue and subcutaneous Fat breakdown and mobilization of adipose tissue also increase, eventually leading to increased body mass.