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目的:建立HPLC法测定蓝玉簪颗粒中三七皂苷R1与人参皂苷Rg_1、Rb_1含量的方法。方法:色谱柱为Agilent C_(18)(250 mm×416 mm,5μm),以乙腈-水为流动相,梯度洗脱;检测波长为203nm;流速为1.0ml·min~(-1);柱温为35℃。结果:三七皂苷R_1在0.56～3.54μg、人参皂苷Rg_1在0.41～8.12μg、人参皂苷Rb_1在0.40～5.31μg范围内线性关系良好,相关系数r均为0.999 9。三七皂苷R1与人参皂苷Rg_1、Rb_1的平均回收率分别是99.45%、99.11%、99.84%;RSD值分别为0.97%、0.56%、0.24%(n=6)。结论:本方法操作简便、可靠,重复性好,可作为蓝簪这颗粒中三七皂苷R_1、人参皂苷Rg_1、Rb_1的含量测定方法。
Objective: To establish a method for the determination of notoginsenoside R1 and ginsenoside Rg_1 and Rb_1 in Lanyuqi granule by HPLC. Method: The chromatographic column was Agilent C 18 (250 mm×416 mm, 5 μm). The mobile phase was acetonitrile-water with gradient elution. The detection wavelength was 203 nm and the flow rate was 1.0 ml·min -1. The temperature is 35°C. RESULTS: The linear relationship between notoginsenoside R1 in 0.56-3.54 μg, ginsenoside Rg-1 in 0.41-8.12 μg and ginsenoside Rb-1 in 0.40-5.31 μg was good, and the correlation coefficient r was 0.999 9. The average recoveries of notoginsenoside R1 and ginsenoside Rg_1 and Rb_1 were 99.45%, 99.11%, and 99.84%, respectively; RSD values were 0.97%, 0.56%, and 0.24% (n=6), respectively. Conclusion: This method is simple, reliable, and reproducible. It can be used as a method to determine the content of notoginsenoside R_1 and ginsenoside Rg_1 and Rb_1 in the granules of Lanjing.