【摘 要】
：
为了提高重组大肠杆菌(Escherichia coli)Rosetta(DE3 )-pET-22-bgl-(kil-Km)的产酶能力,采用单因素试验研究了培养基碳源、氮源及碳氮比(摩尔比)对重组大肠杆菌Rosetta(DE3)
【基金项目】
：
科技部科研院所技术开发研究专项基金;
论文部分内容阅读
为了提高重组大肠杆菌(Escherichia coli)Rosetta(DE3 )-pET-22-bgl-(kil-Km)的产酶能力,采用单因素试验研究了培养基碳源、氮源及碳氮比(摩尔比)对重组大肠杆菌Rosetta(DE3)-pET-22-bgl-(kil-Km)分泌表达重组β-1,3-1,4-葡聚糖酶H(A107-M)的影响.在此基础上,采用Box-Behnken设计法和响应面分析法对以上影响因素进行优化,得出最优培养基成分为(g/L):甘油10.01,酪蛋白胨12.03,酵母粉24,NaCl 10,(NH4)2SO44.77,KH2PO4 2.31,K2 HPO4 12.54.在该优化培养基中培养27 h后胞外酶活达到78.32 U/mL,是基础产酶培养基中酶活的2.84倍.
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