论文部分内容阅读
目的研究新分离噬菌体PF18的生物学特性及其对肺炎克雷伯菌所致小鼠全身感染的疗效。方法调查PF18的噬菌谱,观察其感染肺炎克雷伯菌临床株F18的噬菌斑形态。电镜下明确其形态分类。提取噬菌体PF18的基因组并进行酶切鉴定。观察PF18尾静脉注射治疗全身感染小鼠的生存状态变化。结果 PF18在F18上可形成直径约为5mm完全透明且周围有晕环的噬菌斑,电镜显示其属于有尾噬菌体目,长尾噬菌体科。PF18感染F18的潜伏期为17min,爆发量约为200PFU/细胞。PF18的基因组可被EcoRI、BamHI两种限制性内切酶降解,但不能被HindⅢ,kpnI降解。PF18治疗组小鼠1d内生存率为100%,1周内生存率可达30%,而无治疗对照组小鼠1d能全部死亡,差异有统计学意义(P<0.01)。结论 PF18潜伏期短,爆发量大,且治疗肺炎克雷伯菌所致小鼠的全身感染有一定疗效,有望作为对抗多重耐药肺炎克雷伯菌感染的生物抗菌剂,应对PF18进行深入研究。
Objective To study the biological characteristics of newly isolated bacteriophage PF18 and its effect on systemic infection in mice induced by Klebsiella pneumoniae. Methods The phage spectrum of PF18 was investigated and the plaque morphology of PF18 infected with Klebsiella pneumoniae was observed. Electron microscopy clear its morphological classification. The genome of phage PF18 was extracted and identified by restriction enzyme digestion. To observe the changes of the survival status of mice infected by PF18 tail vein. Results PF18 on F18 formed a diameter of about 5mm completely transparent and halo around the plaques, electron microscopy showed that it belongs to a phage phage phage, phage phage phage. The incubation period of F18 with PF18 was 17 min and the outbreak was about 200 PFU / cell. The genome of PF18 can be degraded by two EcoRI and BamHI restriction enzymes but not by HindIII and kpnI. The survival rate of mice treated with PF18 for 1 day was 100%, and the survival rate was up to 30% in 1 week. However, the mice in control group were all dead at 1 day, with significant difference (P <0.01). Conclusions PF18 has a short incubation period and large eruption volume. It has a certain curative effect in the systemic infection of mice induced by Klebsiella pneumoniae. It is expected that PF18 should be further investigated as a bio-antibacterial against Klebsiella pneumoniae infection.