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前期研究中将主效抗病QTL(qMrdd1)定位在第8染色体分子标记M103.4-M105.3之间,该抗病QTL表现为隐性基因遗传,可降低24.2%~39.3%的发病率。通过分子标记检测,在抗病系NT411(供体亲本)和感病系NT409(轮回亲本)回交多代的群体中,选择7个含有抗性QTL(qMrdd1)的杂合单株自交得到分离群体B1、B2、B3、B4、B5、B6、B7和来源于同一回交后代的两对抗感病近等基因系(B8-R、B8-S和B9-R、B9-S),利用这两对近等基因系与自交系A7110、Q319、CT03、昌7-2、43684、43683、43946组配13对杂交种,在植株整个生育期内无粗缩病病毒接种的条件下,在北京、海南和山东种植不同的材料并对7个分离群体和13对杂交种进行农艺性状调查,结果表明,7个分离群体中各基因型植株以及13对杂交种在株高、穗长、穗行数等农艺性状上差异不显著,qMrdd1基因对玉米产量性状不存在多效性现象,可以应用于抗粗缩病育种。
In the previous study, qMrdd1, a major QD disease marker, was mapped on the M103.4-M105.3 chromosome 8 marker. The QTLs showed recessive genetic inheritance, which reduced the incidence of qMrdd1 by 24.2% -39.3% . By means of molecular marker detection, seven hybrid individuals with resistance QTL (qMrdd1) were self-fertilized in a population of backcross generations of resistant line NT411 (donor parent) and susceptible line NT409 (reincarnated parent) The two pairs of susceptible alleles (B8-R, B8-S and B9-R, B9-S) derived from the same backcross were isolated from populations B1, B2, B3, B4, B5, B6, The two pairs of near-isogenic lines and inbred lines A7110, Q319, CT03, Chang 7-2,43684,43683,43946 group with 13 pairs of hybrids throughout the growth period of the plant without the conditions of virus inoculation, In Beijing, Hainan and Shandong, different materials were planted and the agronomic traits of 7 segregated populations and 13 pairs of hybrids were surveyed. The results showed that there were no significant differences in plant height, ear length, There was no significant difference in agronomic traits between rows and rows. QMrdd1 gene did not have pleiotropy on yield traits in maize, which could be applied to anti-sickening breeding.