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磷脂酰肌醇磷酸5-激酶9(PIP5K9)参与负调控植物碱性/中性转化酶的活性。本研究根据拟南芥PIP5K9基因序列信息,BLAST搜索木薯基因组数据库,找到木薯PIP5K9基因序列信息,利用RT-PCR方法克隆了木薯PIP5K9基因,命名为Me PIP5K9。生物信息学分析结果表明,该基因全长5 421 bp,包含8个外显子和7个内含子,CDS区全长2 496 bp,编码831个氨基酸。蛋白的N端包含8个MORN结构域,C端具有PIPKc结构域。序列比对和系统进化树分析结果表明,Me PIP5K9与麻风树PIP5K9亲缘关系最近,其次为蓖麻。基因表达分析发现,Me PIP5K9在叶片中表达量最高,其次是须根、茎和花,在块根和果中的表达量最低。本研究分离鉴定了木薯PIP5K9基因,分析了该基因的生物学信息及在不同组织中的表达模式,为进一步探讨其调控木薯碱性/中性转化酶的活性奠定基础。
Phosphatidylinositol 5-kinase 9 (PIP5K9) is involved in the negative regulation of plant alkaline / neutral invertase activity. In this study, according to the sequence information of Arabidopsis PIP5K9 gene, BLAST searches the cassava genome database to find out the sequence information of Cassava PIP5K9 gene. The PIP5K9 gene was cloned by RT-PCR and named as Me PIP5K9. The results of bioinformatics analysis showed that the full-length cDNA was 5 421 bp in length and contained 8 exons and 7 introns. The CDS region was 2 496 bp in length, encoding 831 amino acids. The protein contains 8 MORN domains at the N-terminus and PIPKc domain at the C-terminus. Sequence alignment and phylogenetic tree analysis showed that Me PIP5K9 had the closest genetic relationship with Jatropha PIP5K9, followed by Castor. Gene expression analysis showed that Me PIP5K9 had the highest expression level in leaves, followed by fibrous roots, stems and flowers, and lowest in tuberous roots and fruit. In this study, we isolated and identified the cassava PIP5K9 gene, analyzed the biological information of the gene and its expression pattern in different tissues, and laid the foundation for further understanding its regulation of alkaline / neutral invertase activity.