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目的:探讨复方中药制剂对运动大鼠心肌损伤的保护作用机制。方法:96只Wistar大鼠随机分为4组:对照组,服药组,训练组,训练+服药组,每组24只。检测不同运动状态下大鼠心肌组织丙二醛(MDA)、总Ca2+含量及超氧化物歧化酶(SOD)、Ca2+-ATPase、Na+-K+-ATPase活性。结果:①安静状态下,MDA含量、SOD活性训练+服药组与其它各组间差异有统计学意义(P<0.05,P<0.01);②力竭状态下,SOD、总Ca2+含量、Ca2+-ATPase训练+服药组与其它各组间差异有统计学意义(P<0.01);③力竭恢复12h状态下,SOD、总Ca2+含量、Na+-K+-ATPase训练+服药组与其它各组间差异有统计学意义(P<0.01,P<0.05)。结论:复方中药能有效清除大鼠心肌内自由基,提高大鼠心肌组织抗氧化能力;抑制心肌细胞内钙超载,保护心肌细胞的完整性;有氧耐力训练与复方中药联合使用效果更为明显。
Objective: To investigate the protective effect of Compound Chinese Herbal Medicine on myocardial injury in rats. Methods: 96 Wistar rats were randomly divided into 4 groups: control group, medication group, training group, training + medication group, 24 rats in each group. The activities of malondialdehyde (MDA), total calcium (Ca2 +), superoxide dismutase (SOD), Ca2 + -ATPase and Na + -K + -ATPase in myocardial tissue of rats were measured under different exercise conditions. Results: (1) Under the condition of exhaustive exercise, the content of MDA and the activity of SOD activity + the difference between the medication group and other groups were statistically significant (P <0.05, P <0.01) (P <0.01); (3) After exhaustive recovery of 12h, SOD, total Ca2 + content, Na + -K + -ATPase training + medication group and other groups were significantly different There was statistical significance (P <0.01, P <0.05). Conclusion: Compound traditional Chinese medicine can effectively eliminate free radicals in rat myocardium, improve myocardial antioxidant capacity; inhibit myocardial calcium overload, protect the integrity of cardiomyocytes; aerobic endurance training and compound Chinese medicine combined effect is more obvious .