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目的探讨新型阻燃剂磷酸三(2-氯乙基)酯(TCEP)的毒性效应,为TCEP的安全性评价提供依据。方法本实验以稀有鮈鲫(Gobiocypris rarus)为实验生物,采用半静态实验方法,分别进行96 h急性毒性和28 d慢性毒性实验。对稀有鮈鲫肝脏及脑组织中SOD和GSH-Px酶活性进行测定。结果 TCEP对稀有鮈鲫的96 h半致死浓度LC50为136.9(111.6~167.9)mg/L;经过TCEP暴露28 d后,稀有鮈鲫肝脏及脑组织中SOD和GSH-Px酶活性变化测定结果显示,与对照组相比,暴露组SOD和GSH-Px的活性均受到明显抑制,且随着TCEP的暴露浓度增加其抑制作用均显著增强(P<0.05)。结论 TCEP暴露可以诱发稀有鮈鲫毒性作用,并对水生生物具有明显的生态毒性效应。
Objective To investigate the toxic effects of a novel flame retardant, tris (2-chloroethyl) phosphate (TCEP), to provide a basis for the safety evaluation of TCEP. Methods In this experiment, Gobiocypris rarus was used as the experimental organism and the semi-static experimental method was used to conduct the 96 h acute toxicity and 28 d chronic toxicity experiments respectively. The activities of SOD and GSH-Px in the liver and brain of Rana nigromaculata were determined. Results The LC50 of TCEP at 96 h for Rare-sized Carassius auratus was 136.9 (111.6 ~ 167.9) mg / L. After 28 days exposure to TCEP, the changes of SOD and GSH-Px activities in liver and brain Compared with the control group, the activities of SOD and GSH-Px in the exposed group were significantly inhibited, and the inhibitory effect was significantly enhanced with the exposure concentration of TCEP (P <0.05). Conclusion TCEP exposure can induce toxic effects of Carassius auratus, and has obvious ecotoxicological effects on aquatic organisms.