以葡萄孢属TB-3菌株为出发菌株制备其原生质体．在纤维素酶浓度为20g／L，蜗牛酶浓度为3g／L的酶解系统中，25℃酶解3h，其原生质体制备数可达67×105mL-1，在KYM上的再生率为46．1％，在KPDA上的再生率为33．6％，经过3轮原生质体紫外线诱变后回复再生，及对大量再生突变株进行发酵筛选，获得高产稳定株TB－3H8，其发酵液中ABA的质量浓度可达1．4g／L Protoplasts were prepared from Botrytis TB-3 strain. In enzymolysis system with cellulase concentration of 20 g / L and snail enzyme concentration of 3 g / L, the number of protoplasts was up to 67 × 105 mL-1 after hydrolysis at 25 ℃ for 3 h, and the regeneration rate on KYM was 46 .1%, the regeneration rate on KPDA was 33.6%. After three rounds of protoplast UV mutagenesis and regeneration, and a large number of regenerated mutant strains were fermented and screened to obtain high-yielding stable strain TB-3H8, the fermentation broth ABA mass concentration of up to 1.4g / L