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目的:构建小趋化因子VCC-1的慢病毒表达载体并研究其对人胶质瘤细胞株U251增殖的影响。方法:以人结肠癌细胞为模板扩增VCC-1基因,并连入pMX载体,挑取测序正确的克隆在293T细胞中表达,并用realtime-PCR和Western blot进行验证。结果:成功构建了人VCC-1的慢病毒表达载体,并感染了星形胶质瘤细胞系U251。结论:证明了VCC-1基因在胶质瘤细胞的增殖过程中起促进作用,并为后续研究VCC-1和胶质瘤形成发展和浸润的机制提供了良好的基础。
OBJECTIVE: To construct a lentiviral vector expressing small chemokine VCC-1 and study its effect on the proliferation of human glioma cell line U251. Methods: Human colon cancer cells were used as template to amplify VCC-1 gene and inserted into pMX vector. The correct clones were picked out and transfected into 293T cells. Real-time PCR and Western blot were used to verify the results. Results: The lentiviral vector of human VCC-1 was successfully constructed and infected the astrocytoma cell line U251. CONCLUSIONS: VCC-1 gene is found to play a catalytic role in the proliferation of glioma cells and provide a good basis for further studies on the mechanism of VCC-1 and glioma formation and invasion.