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目的建立H37Rv结核分支杆菌诱导的兔脊柱结核模型。方法选择未致敏处理的新西兰大白兔48只,在第5腰椎近椎间盘处钻孔,并填充明胶海绵,注射0.5 mg/0.1 m L结核菌悬液,术后观察新西兰大白兔一般情况,并用影像学、组织病理学、细菌学等检查对兔脊柱结核模型进行评估。结果受H37Rv结核菌株感染后的新西兰大白兔,局部反应较明显,全身反应较轻。48只兔中38只兔完成实验,10只因死亡、术后截瘫被淘汰,15只兔分别于术后第5、6周出现进食欠佳、消瘦,但生命体征平稳,其中4只术区局部肿胀,6只出现下肢运动障碍;其余23只兔术后进食较好,体质量未见明显变化。术后3个月行外科手术,暴露致病兔椎体,38只兔中29只兔椎体可见虫噬样改变,9只兔椎体未见明显变化。术中取脓肿形成的兔脓液培养示结核分枝杆菌生长;取周围软组织、脓壁行苏木精-伊红色(HE)染色,示坏死灶形成、较多炎细胞聚集、淋巴细胞、较少上皮样细胞,骨结构紊乱或消失。建立模型成功率为76.3%(29/38)。结论使用0.5 mg/0.1 m L的H37Rv标准菌株感染的新西兰白兔结核模型建立成功。
Objective To establish a model of spinal tuberculosis induced by Mycobacterium tuberculosis H37Rv in rabbits. Methods Forty-eight New Zealand white rabbits without sensitization were drilled at the 5th lumbar intervertebral disc, filled with gelatin sponge and injected with 0.5 mg / 0.1 m L suspension of M. tuberculosis. The general condition of New Zealand white rabbits was observed and compared with Imaging, histopathology, bacteriology and other tests to assess the rabbit spinal tuberculosis model. Results New Zealand white rabbits infected with H37Rv tuberculosis strains showed obvious local reactions and mild systemic reactions. Thirty-eight rabbits of 38 rabbits completed the experiment, and 10 died of paraplegia after operation. Fifteen rabbits were poorly fed and lost weight at the 5th and 5th week after operation, but their vital signs were stable. Four rabbits Local swelling, 6 cases of lower extremity dyskinesia; the remaining 23 rabbits eat better, no significant change in body weight. Surgical operation was performed 3 months after operation to expose the vertebrae of the pathogenic rabbit. The worm-like changes of 29 rabbits in 38 rabbits were observed, and no significant changes were observed in 9 rabbits. Intraoperative abscess formation in rabbit pus culture showed Mycobacterium tuberculosis growth; take the surrounding soft tissue, pus wall line hematoxylin-eosin (HE) staining, showing necrosis, the formation of more inflammatory cells, lymphocytes, compared with Less epithelioid cells, bone structure disorder or disappear. The model success rate was 76.3% (29/38). Conclusion New Zealand white rabbits infected with 0.5 mg / 0.1 m L H37Rv standard strain were successfully established.