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目的探讨双特异性抗体用于白血病自体干细胞移植体外净化的可能性。方法以抗ＣＤ３／ＣＤ４５双特异性抗体激活外周血单个核细胞，以ＨＬ６０为靶，用ＬＤＨ方法测定杀伤效应。结果靶效比例不同时，抗ＣＤ３／ＣＤ４５激活的效应细胞对ＨＬ６０细胞都表现出杀伤效应。结论抗ＣＤ３／ＣＤ４５可能用于骨髓／造血干细胞移植的体外净化。
Objective To investigate the possibility of using bispecific antibodies for the purification of leukemia autologous stem cells in vitro. Methods Peripheral blood mononuclear cells were activated by anti-CD3/CD45 bispecific antibody. The killing effect was measured by LDH method. Results When the ratio of target effect was different, anti-CD3/CD45 activated effector cells showed killing effect on HL60 cells. Conclusion Anti-CD3/CD45 may be used for the in vitro purification of bone marrow/hematopoietic stem cell transplantation.