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The total effective spin-exchange relaxation of naturally abundant Rb in a K-Rb-21Ne comagnetometer is analyzed, and the results show that the coexistence of 87Rb and 85Rb isotopes in the same volume can lead to a large extra spin-exchange broadening compared to pure 87Rb. This broadening mainly comes from the contribution of the equivalent reduction in the Rb spin-exchange rate. On this basis, an approximate relaxation model is proposed and experimentally demonstrated to be more accurate than that from a previous work. This study also provides a method for determining the properties of alkali-metal vapor cells.