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初步研究了分离自石榴果实中的一株枯草芽孢杆菌Bacillus subtilis所产生的挥发性物质对几种常见林果病原菌的抑菌作用。发现该挥发性物质对不同病原菌菌丝生长具有一定的抑制作用,其中对杨树溃疡病菌Botryosphaeria dothidea的抑制率达60%。将不同病原菌株与该挥发性物质互作后的菌丝形态与正常菌丝进行比较,结果表明:处理后的杨树溃疡病菌、石榴疮痂病菌B.dothidea、苹果轮纹病菌B.dothidea及葡萄白腐病菌Coniothyrium diplodiella的菌丝分支增多,扭曲,菌丝顶端和中部细胞膨大;杨树腐烂病菌Valsa sordida及桑紫纹羽病菌Helicobasidium purpureum的菌丝变粗;合欢枯萎病菌Fusarium oxysporum的菌丝变细;苹果霉心病菌Trichothecium roseum菌丝形态无明显变化。同时发现,不同病原菌菌丝色素产生的速度均受到了抑制。表明该枯草芽孢杆菌所产生的挥发性物质对不同病原菌的抑菌效果和作用方式不同。
The preliminary study on the bacteriostasis of volatile compounds from a strain of Bacillus subtilis isolated from pomegranate fruit on several common pathogenic fungi. It was found that the volatile compounds could inhibit the growth of mycelium of different pathogens to a certain degree, and the inhibition rate to Botryosphaeria dothidea was about 60%. The results of comparison of the mycelial morphology between the different pathogenic strains and the volatile substance after the interaction with the normal mycelium showed that the treated poplar canker, B.dothidea, B.dothidea and grapes Mycorrhizal branches of Coniothyrium diplodiella increased, twisted, and mycelial tip and central cells expanded; the mycelia of Valsa sordida and Helicobasidium purpureum became thick; the hyphae of Fusarium oxysporum Fine; apple mold heart bacteria Trichothecium roseum mycelium no significant changes in morphology. Also found that different pathogens mycelial pigment production rate have been inhibited. The result showed that the bacterium produced by Bacillus subtilis had different antibacterial effect and action on different pathogenic bacteria.