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土壤微生物群落的组成一直是土壤学、微生物学和生态学研究的热点问题。我国在这方面的研究处于国际前列,越来越多的研究成果在国际重要刊物上发表。而磷脂脂肪酸(PLFA)法在土壤微生物群落分析中占有举足轻重的地位,国内外学者都热衷于使用该方法。但是PLFA法的使用仍存在一些不足的地方,需要研究学者们慎重使用。本文综述了国际上相关研究,概述了PLFA方法使用的发展历史,应用及挑战。总结了使用和数据解读时需要注意的问题,整理了PLFA法相关的生物标记以及与新方法结合的设想,方便以后研究的开展。
The composition of soil microbial community has always been a hot issue in soil science, microbiology and ecology. China’s research in this area is at the forefront of the world, and more and more research findings are published in major international journals. Phospholipid fatty acid (PLFA) method plays an important role in the analysis of soil microbial community, and scholars at home and abroad are keen to use this method. However, there are still some shortcomings in the use of the PLFA method, which requires careful study by scholars. This article summarizes the relevant international studies and summarizes the development history, applications and challenges of the use of PLFA methods. Summarized the use and interpretation of the data need to pay attention to the problem, sorted out the PLFA method related to biomarkers and ideas combined with the new method to facilitate future research.