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运用Nested-PCR技术对来自广西部分市、县的40个黄皮和九里香进行柑桔黄龙病(CitrusHuanglongbing,HLB)病原检测,结果表明:从部分黄皮和九里香样品抽提的DNA中,可扩增出一条特异性的400bp条带,说明广西种植的黄皮和九里香均可感染黄龙病;在柑桔黄龙病疫区,黄皮和九里香黄龙病感染率分别为35.0%和40.0%,黄龙病感染率已相当高。为了减少柑桔黄龙病的发生,有利于对柑桔黄龙病进行综合防控,不宜在柑桔种植区栽种黄皮和九里香,铲除已感染黄龙病的植株。
Nested-PCR was used to detect the pathogen of Citrus Huanglongbing (HLB) in 40 Wampee and Jiulixiang from some cities and counties in Guangxi. The results showed that from the DNA extracted from some samples of Wampee and Jiu Li Xiang, A specific 400 bp band was amplified, indicating that Huangpi and Jiulixiang planted in Guangxi could be infected with Huanglongbing. In the citrus Huanglongbing disease, the infection rates of Huanghuang and Jiulixiang were 51.0% and 40.0% respectively, , Huanglongbing infection rate has been quite high. In order to reduce the incidence of citrus yellow dragon disease, which is conducive to comprehensive prevention and control of citrus yellow dragon’s disease. It is not advisable to plant yellow and Ji Li Hong in citrus growing areas to eradicate the plants that have already infected Huanglongbing.